Table 1. Antibacterial activity of 2-AFAs and other analogues against Gram-positive and Gram-negative bacteria at 37°C after 18-24 ha.
| Compound | MIC/ IC50 ± SEM (μg/mL) | |||||
|---|---|---|---|---|---|---|
|
| ||||||
| S. aureus (ATCC 29213) | S. saprophyticus (ATCC 15305) | B. cereus (ATCC 10876) | E. coli (ATCC 25922) | K. pneumoniae (ATCC 13883) | P. aeruginosa (ATCC 27853) | |
| 2-HDA | 15.6/6.2 ± 0.9 | 15.6/8.8 ± 0.1 | 31.3/17.2 ± 1.0 | >1,000 | 7.8/6.1 ± 0.1 | 125/87.8 ± 1.1 |
| 2-ODA | 15.6/8.1 ± 1.0 | 125/68.8 ± 3.5 | 15.6/11.9 ± 0.8 | >1,000 | 7.8/5.6 ± 0.2 | >1,000 |
| 2-ICA | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 |
| 2,6-HDA | 31.3/14.1 ± 0.1 | 62.5/21.5 ± 0.6 | 62.5/46.1 ± 0.3 | >1,000 | 62.5/ 31.2 ± 0.5 | 250/132.3 ± 3.5 |
| 2,9-HDA | 250/94.0 ± 0.9 | 125/61.5 ± 1.2 | 62.5/34.2 ± 0.3 | >1,000 | 250/135.4 ± 0.7 | >1,000 |
| 2-HDOTHP | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 |
| 2-ODOTHP | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 |
| 2-HDOH | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 |
| 2-ODOH | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 | >1,000 |
| METH | 0.06/0.03 ± 0.01 | 0.12/0.06 ± 0.01 | 62.5/38.2 ± 0.7 | >1,000 | 0.98/0.36 ± 0.01 | >1,000 |
| CIPRO | 0.25/0.14 ± 0.01 | <0.002 | 0.05/0.03 ± 0.01 | <0.008 | 0.24/0.13 ± 0.01 | 0.24/0.11 ± 0.01 |
a
Experiments were performed in triplicate (N =3). Meth, Methicillin, Cipro, Ciprofloxacin.