. 2014 Jun 6;9(6):e98259. doi: 10.1371/journal.pone.0098259

Table 1. Characteristics of included studies.

First Author	Year	Country of population	Sample size	Histology	Stage	First- antibody	Neutrophils location	Cut-off criteria	Cut-off value	Follow-up time	Hazard ratios	Survival analysis
Rosario Alberto Caruso	2002	Italy	273	Gastric	Ib- IV	- (HE)	Intratumoral	Mean	>10cells/20HPF	NM	Report^a	OS
Frede Donskov	2006	Denmark	85	RCC	NM	CD66b	Intratumoral	Median	0 cells/mm²	Median:57m(32–73 m)	Report^a, Author^a	OS
Hanne Krogh Jensen	2009	Denmark	121	RCC	I-IV	CD66b	Intratumoral	Median	0 cells/HPF	Median:124 m (74–194 m)	Report^a+b+c, Author^a	RFS, OS, CSS
Sokratis Trellakis	2010	Germany	40	HNC	III -IV	CD66b	Intratumoral	NM	Low: −,+; High: ++,+++	Median:69 m (43–124m)	SR ^a	OS
Dong-Ming Kuang	2010	China	200	HCC	I-III	CD15	Intratumoral, Peritumoral, Stromal	Median	Intratumoral:4; Peritumoral:54; Stromal:10	NM	SR^a+d, Survival curve^a+d	DFS, OS
Yi-Wei Li	2011	China	281	HCC	I-III	CD66b	Intratumoral Peritumoral	Fixed	Intratumoral:70%, Peritumoral:50%	Median:29 m (1.5–83m)	Report^a+c, Survival curve^a+c	RFS, OS
Lih-Chyang Chen	2012	China	140	HNC	NM	- (HE)	Intratumoral	Mean	10 neutrophils/ 100 epithelial cells	NM	Survival curve^c	RFS
Qiang Gao	2012	China	240	HCC	NM	CD66b	Intratumoral	Median	12 cells/ mm²	NM	Report^c	RFS
Fang-Ming Gu	2012	China	123	ICC	I/II/IIIa/ IIIc	CD66b	Intratumoral, Peritumoral	Median	86 cells/ mm²	Median:13m (4–111m)	Report^a	OS
Marius Ilie	2011	France	632	NSCLC	I – III	CD66b	Intratumoral	Median	49 cells/mm²	Median:30m (0–112 m)	Survival curve^a	OS
Trine O. Jensen	2011	Denmark	186	Melanoma	I/II	CD66b	Intratumoral	Fixed	0 cells/2 HPF	Median:12.2y (10.4–14.2 y)	Report^a+b+c	RFS,OS, CSS
Hui-Lan Rao	2012	China	229	CRC	I-IV	CD66b	Intratumoral	Mean	60 per TMA spot	Average:55.4m Median:60.0m (0.5–98 m)	Report^a, Survival curve^a	OS
CH Richards	2012	UK	130	CRC	NM	- (HE)	Peritumoral	Median	High: score 2–3, Low: score 0–1	Median:105m (55–163m)	DE^b	CSS
Jing-jing Zhao	2012	China	115	Gastric	I-IV	CD15	Intratumoral	Median	21.60 cells/HPF	NM	Report^a	OS
Shao-Lai Zhou	2012	China	323	HCC	NM	CD66b	Intratumoral	NM	Hgih, Low	NM	Report^a+c	OS, RFS
Andreas Carus	2013	Denmark	101	CC	IB-IIA	CD66b	Intratumoral, Peritumoral, Stromal	Median	Intratumoral:23.2cells/mm², Peritumoral:53.1cells/mm², Stromal:28.3cells/ mm²	NM	Author^c, Report^c	RFS
Andreas Carus	2013	Denmark	335	NSCLC	I–IIIA	CD66b	Intratumoral, Peritumoral, Stromal	Median	Intratumoral:8.7cells/mm², Stromal:21.0 cells/ mm²	NM	Author^a+c	OS, RFS
Claudia A. Dumitru	2013	Germany	97	HNC	NM	CD66b	Intratumoral	Median	NM	NM	Survival curve^a	OS
Claudia A. Dumitru	2013	Germany	83	HNC	I-IV	CD66b	Intratumoral	NM	High: medium and strong, Low: negative, weak	NM	Report^a	OS
Y Ino	2013	Japan	212	PDC	NM	CD66b	Intratumoral	Median	NM	Median:18.8m (2.6–201m)	Report^a+d	OS, DFS

Abbreviations: Gastric: gastric carcinoma; RCC: renal cell carcinoma; HNC: head and neck carcinoma; HCC: hepatocellular carcinoma; ICC: intrahepatic cholangiocarcinoma; NSCLC: non-small-cell lung cancer; CRC: colorectal carcinomas; CC: cervical cancer; PDC: pancreatic ductal carcinoma; HE: hematoxylin-eosin staining; OS: overall survival; CSS: cancer-specific survival; RFS: recurrence-free survival; DFS: disease-free survival; NM: not mentioned; a: OS; b: CSS; c: RFS; d: DFS; m: months; y: years; DE: data extrapolated; SR: systematic review [36].