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. 2014 Jun 6;9(6):e97806. doi: 10.1371/journal.pone.0097806

Figure 6. Onset expression of gfp mRNA during somitogenesis in Tg(gtshβ:GFP).

Figure 6

(A) WISH analysis of gfp (arrowheads) in 15, 16 and 17 hpf embryos, dorsal-lateral view. (B) Transverse sections of the embryos in A. (C) Higher magnification of boxed regions of B. (D) Cell boundary staining by the RFP-CVLS chimera protein on transverse sections of Tg(gtshβ:GFP) embryos. The gfp-positive cells are outlined by black dash lines. The tubular structures are outlined by white dash lines. Arrows indicate the lumen. nc: neural cord; n: notochord; pt: pronephric tubule. Bar = 10 µm.