Table 2. Results of the in vitro PhzO activity assay.
NAD(H) | NADP(H) | Co2+ | Mn2+ | Mg2+ | Fe3+ | PCA | 2-OH-PCA | 2-OH-PHZ | |
1 | − | − | − | − | − | − | + | ND | ND |
2 | − | + | − | − | − | − | + | ND | ND |
3 | − | + | + | − | − | − | + | ND | ND |
4 | − | + | − | + | − | − | + | ND | ND |
5 | − | + | − | − | + | − | + | ND | ND |
6 | − | + | − | − | − | + | + | D | D |
7 | + | − | − | − | − | − | + | ND | ND |
8 | + | − | + | − | − | − | + | ND | ND |
9 | + | − | − | + | − | − | + | ND | ND |
10 | + | − | − | − | + | − | + | ND | ND |
11 | + | − | − | − | − | + | + | ND | ND |
12 | − | − | − | − | − | − | − | ND | ND |
+ represents the addition of the specified metal ion, the final concentration of metal ion was 1 mM; the final concentration of PCA was 200 mg/L.
D, detectable.
ND, not detectable.