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. 2014 Jun 6;9(6):e97739. doi: 10.1371/journal.pone.0097739

Figure 4. TTK overexpression promotes cell proliferation and resistance to sorafenib in HCC cells.

Figure 4

(A) Cell growth curves were described according to cell viability for five days (right panel), when recombinant pcDNA3.1-TTK and empty vector were transiently transfected into Huh7 and HepG2 cells respectively, confirmed by western blotting assay, where β-actin was used as an internal reference (left panel). (B) Representative dishes of anchor-dependent colony formation of Huh7 and HepG2 cells were shown, where these cells were transfected with recombinant pcDNA3.1-TTK and empty vector (left panel). The numbers of colonies were counted and statistically analyzed using a two-tailed t test (right panel). (C) The cytotoxic effects of sorafenib at different concentrations (0.5, 1, 2, 5, 10 µM) on cell viability was shown, where pcDNA3.1-TTK and empty vector were transfected into Huh7 (upper panel) and HepG2 (lower panel) cells, respectively. (D) As shown were the microscopic fields of anchorage-independent colony formation assay of Huh7 and HepG2 cells treated with 1 µM sorafenib, upon TTK overexpression (left panel). The numbers of colonies were counted and statistically analyzed using a two-tailed t test (right panel). *, P<0.05; **, P<0.01.