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. 2014 Jun 6;9(6):e99052. doi: 10.1371/journal.pone.0099052

Figure 2. ER-mediated Vav1 expression.

Figure 2

(A) MCF7 and T47D cells were treated with E2 (10−7 mol/L) or DMSO for 24 h. The relative level of vav1 mRNA was determined by qRT-PCR and was presented by the ratio of vav1 mRNA of E2-treated samples to that of DMSO-treated control samples and presented as y-axis. The data represented the mean value±S.D. of three independent experiments. (B and C) MCF7 and T47D cells were exposed to E2 (10-7 mol/L) for 0 to 72 h (B), or increasing concentration of E2 for 48 h (C). (D and E) MCF7 and T47D cells were pre-treated with ICI 182,780 (4×10−7 mol/L) (D) or increasing concentration of Tamoxifen for 30 min (E) before adding E2 (10−7 mol/L) for 48 h. The DMSO treatment was used as a solvent control. The Vav1 expression in above treated samples was analyzed by Western Blot with anti-Vav1 antibody, with tubulin as protein loading control. The bar chart below each example blot represents the normalized protein level of Vav1 to Tubulin of three independent experiments. The DMSO treatment was set as 1 to indicate the basal level of Vav1 expression. “**” indicates P<0.01 versus DMSO treatment and “a**” indicates P<0.01 versus E2 treatment by unpaired student T test.