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. 2014 Jun 6;9(6):e99118. doi: 10.1371/journal.pone.0099118

Figure 5. HDAC4-NFAT1/NFAT3 complex contribution to regulation of PMCA alternative splicing in PC12 cells.

Figure 5

HDAC/NFAT involvement in regulation of alternative splicing of PMCA transcripts was analyzed by qPCR-chromatin immunoprecipitation. The analysis was performed for the splicing at site A and at site for four PMCA isoforms and the PCR products were migrated in 1% agarose gels (A). The qPCR data, were expressed as fold of change (2−ΔΔC) calculated from the difference: ΔCT of output (immunoprecipitated DNA with HDAC/NFATs) – ΔCT of input (total DNA) and statistics were calculated according to nonparametric paired Wilcoxon signed rank test at 95% confidence, where PMCA2-deficient cells (_2) or PMCA3-deficient cells (_3) were compared to control cells assigned to y = 1 value (B). Symbols: control cells (C), PMCA2-deficient cells (_2), PMCA3-deficient cells (_3), n = 3.