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. 2014 Jun 6;9(6):e98972. doi: 10.1371/journal.pone.0098972

Figure 1. Nampt expression is upregulated by ischemic preconditioning (IPC).

Figure 1

A, The IPC protocol for wild-type mice. Mice on C57BL/6 background were subjected to 6 cycles of 3 minutes of ischemia plus 3 minutes of reperfusion. Sham groups were subjected to open chest surgery only. Arrows indicate the timing of biochemical analyses. B, Nampt mRNA expression 8 hours and 24 hours after IPC was determined by quantitative RT-PCR. n = 4. C, Nampt protein expression 20 minutes and 24 hours after IPC was determined by Western blot. D, Nampt protein expression with or without IPC in Nampt +/− mice and their wild-type littermates. E-G, Nampt +/− and littermate wild-type mice were subjected to IPC as shown in A. Five minutes or 24 hours after IPC, the mice were subjected to ischemia (30 minutes)/reperfusion (24 hours) (I/R). Some mice were subjected to I/R without IPC. Infarct size/AAR (E), AAR (F) and % reduction in infarct size compared to those without IPC (G) are shown. In E and F, ## p<0.01 vs. wild-type littermates subjected to the same surgery. n = 4 to 8. In G, ## p<0.01 vs. wild-type littermates subjected to I/R 5 minutes after IPC. In B, E, F and G, n.s., not significant; * p<0.05, ** p<0.01.