Figure 2. NAD+ content in the heart is reduced in Nampt +/− mice.
A and B, Heart homogenates were prepared from Nampt +/− mice and their wild-type (Wt) littermates. A, NAD+ and NADH contents. B, NAD+/NADH ratio. C-E, Mice were subjected to sham procedure or IPC, as shown in Figure 1A. Mice were then subjected to either 30 minutes ischemia or sham operation 5 minutes after the sham procedure or either 5 minutes or 24 hours after IPC. C, NAD+ contents. D, NADH contents. E, NAD+/NADH ratio. In A-E, n = 4. n.s., not significant; * p<0.05, ** p<0.01.