Table 3. Genomic islands.
Island | Chromosomal Coordinatesa | Length (bp) | att b | Kpn HSc | RNA gene target | Gene contentd | Additional supporte | Notef |
Kpn21L | 583102–604583 | 21482 | Yes | No | tRNA-Leu | Restriction | Islander | Tandem with Kpn11L |
Kpn11L | 604583–615770 | 11188 | Yes | Yesg | tRNA-Leu | Phage | Islander, PHAST | Damage; Tandem with Kpn21L; Enterobacteria phage P4 |
Kpn49R | 1296810–1345944 | 49135 | Yes | Alth | tRNA-Arg | Phage, gpII intron | Islander, PHAST | Cronobacter phage ENT47670 |
Kpn38RybB | 1823069–1785570 | 37500 | Yes | Yesi | RybB | Phage, gpII intron | PHAST | Enterobacteria phage Fels 2 |
Kpn29S | 1966140–1994683 | 28544 | Yes | No | tRNA-Ser | Phage | Islander | |
Kpn23SapB | 2286456–2309756 | 23301 | Yes | No | No | Phage, integron, gpII intron | ||
Phast.2 | 2342484–2388101 | 45618 | No | Yesj | No | Phage, gpII intron | PHAST | Salmonella phage Fels 1 |
Kpn40GuaA | 3969748–4010194 | 40447 | Yes | No | No | Phage, gpII intron | PHAST | Salmonella phage SPN1S |
Kpn37X | 4129454–4166020 | 36567 | Yes | No | tmRNA | Phage | Islander, PHAST | Salmonella phage RE-2010; also called tmGI_Kp35 (JF764793) |
Kpn55F | 4603724–4658623 | 54900 | Yes | Yesk | tRNA-Phe | T4SS, ParAB | Islander | Damage |
Kpn16Fis | 4919120–4935114 | 15995 | Yes | Yesl | No | Phage | PHAST | Salmonella phage ST64B |
Islands integrated into RNA genes are assigned the orientation of the target gene; remaining islands are arbitrarily assigned the genomic orientation.
Putative att sites identified at each flank.
Status in the closely related K. pneumoniae HS11286 chromosome; No/Yes, absent/present in HS11286; Alt, alternative island in HS11286 at the Kpn2146 island site.
The PHAST islands are strongly phage-like; we note where other islands have proteins suggestive of phage function, and other features of interest.
All islands had phyloblocks support.
Damage denotes loss of an acceptor stem nucleotide in the tRNA gene fragment [31]; phage names are the closest relatives determined by PHAST.
CP003200.1/581777-594029; with IS903 inserted at position 4245 with 9-bp DR; directly at tRNA-Leu att site with no intervening Kpn21L.
50403-bp alternative island at CP003200.1/1288317-1338720 with same initial 1989 bp as Kpn49R.
CP003200.1/1778319-1808718; 2 mismatches; with 7201 bp deleted from peg.320 to 3′ end of gpII intron.
CP003200.1/2277469-2325549; with ISEc22 at position 15471 with 8-bp DR.
CP003200.1/4502805-4558770; with IS903B insert at position 9777 with 9-bp DR.
CP003200.1/4819193-4835187; no mismatches.