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. 2014 Jun 6;9(6):e98835. doi: 10.1371/journal.pone.0098835

Figure 6. IFNT induces STAT1 and STAT3 activation in BMDMs.

Figure 6

(A) The phosphorylation of signal transducer and activator of transcription 3 (pSTAT3) and (B) pSTAT1 in BMDMs was measured by flow cytometry after 90-min of LPS (100 ng/mL) or IL-4 (20 ng/mL) stimulation (n = 3). (C) The expression of interferon regulatory factor 9 (IRF9) was analyzed by qRT-PCR after 48-h LPS (100 ng/mL) or IL4 stimulation (20 ng/mL); n = 3. Data are presented as mean ± SEM. *P<0.05, **P<0.001.