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. 2014 Jun 6;9(6):e99203. doi: 10.1371/journal.pone.0099203

Figure 1. PBMC recognition of TB10.4 and IVE-TB antigen Rv2034.

Figure 1

PBMC from a PPD+ donor were stimulated with different stimuli for 6 days and IFN-γ production (pg/ml) was determined in the supernatants. Both TB10.4 protein (10 µg/ml) (A) and Rv2034 protein (10 µg/ml) (B) were analyzed as well as control mitogen PHA and Mtb derived PPD (A and B). Medium values (unstimulated PBMC) were subtracted. IFN-γ concentrations were determined from triplicate-pooled supernatant. A cut-off value was set arbitrarily at 100 pg/ml.