Figure 1. Panxs 1 and 2 are present in mouse T cells and secondary lymphoid organs. (A) RT-PCR products generated with specific primers for Panx1, Panx2 and Panx3 were obtained from RNA extracts of T cells isolated from lymph nodes of Balb/c mice and C₂C₁₂ cells used as positive control. Panx1 amplicon was detected from RNA purified from T cells and C₂C₁₂ cells at the expected electrophoretic mobility (∼280 bp). Panx2 amplicon was also present in both samples (∼270 bp). However, Panx3 amplicon was detected in C₂C₁₂ cells but not in T cells. No amplicons were detected in samples having RNA-DNase or water (not shown) that were used as DNA-contamination controls for mRNA isolation and PCR amplification control, respectively. (B) Immunoblot of Panx1 in total homogenates of mouse blood, thymus, axillar lymph nodes and spleen, as well as freshly isolated T cells obtained from lymph nodes of BALB/c mice and Jurkat cells. C₂C₁₂ cells and mouse brain were used as positive controls. One to four main bands reactive to anti-Panx1 antibody were detected at 45–50 kDa and 90 kDa. (C) Immunoblot analysis of Panx2 in total homogenates of spleen, thymus and lymph node. Brain was used as Panx2 positive control. Panx2 reactive bands were detected mainly between 90–110 kDa.