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Hydrogen-bonded water molecules near the 2-deoxy-2-fluoro-β-maltosyl intermediate of the E423A mutein of GlgE (PDB entry 4CN4). The orientation shown is similar to that in Scheme 1 with subsites −1 and −2 labeled. Superimposed on the coordinates is the final 2mFobs – DFcalc, αcalc electron density map calculated at 2.4 Å resolution.
