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. 2014 May 23;19(1):28. doi: 10.1186/2047-783X-19-28

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Copyright © 2014 Wang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Electron microscopy. (A) Normal skeletal muscle obtained after severing the rat limb but before freezing. Regularly arranged myofibrils, clear z-lines, dense mitochondrial cristae, and intact mitochondrial membranes without swelling were seen. (B) Skeletal muscle of a failed replantation after cryopreservation. Images were obtained four hours after reperfusion. Some myofibrils were ruptured with unclear z-lines, the mitochondria exhibited maximal swelling, crests were disarrayed, and parts of the myofibrils showed vacuolation.