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. 2014 May 22;14:142. doi: 10.1186/1471-2229-14-142

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Copyright © 2014 Sun et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Northern blotting analysis of intriguing wheat miRNAs across various tissues and the expression of miRNAs abundant in grain at different time points in developing grains. (A) Normalized expression levels of miRNAs, as revealed by Illumina sequencing reads. (B) Validation of the expression miRNA patterns displayed in (A) through northern blotting assays. (C) Northern blotting analysis of miRNAs in the embryo and endosperm during the development of grains. The 5S rRNA/tRNA bands were visualized via ethidium bromide staining of polyacrylamide gels, which served as a loading control. (D) The expression of tae-miR3117b and tae-miR3061a in the embryo and endosperm during the development of grains were determined by RT-PCR. DG: dry grain; GSE: germinating seed embryos; SH: shoots; SL: seedling leaves; SR: seedling roots; SJ: stems in the jointing stage; YS5: 0–5 mm young spikes; YS15: 10–15 mm young spikes; FL: flag leaves; GRA4: grains 4 days after pollination; GRA8: grains 8 days after pollination; GRA12: grains 12 days after pollination; GRA15: grains 15 days after pollination; EN15: endosperm 15 days after pollination; EM15: embryos 15 days after pollination; EN20: endosperm 20 days after pollination; EM20: embryos 15 days after pollination; EN28: endosperm 28 days after pollination; EM28: embryos 28 days after pollination.