Table 1.
Condition | Mappable reads | rRNA | RPKM background transcription | Significant active genes (p ≤ 0.05) | % hypotheticals in active genes | Significant active hypotheticals (p ≤ 0.05) |
||
---|---|---|---|---|---|---|---|---|
Genome | Plasmid | Total | ||||||
LB (SOLiD) | 1,990,326 | 46,497 | 2,036,823 | 13.0% | 0.2 | 4,557 | 29.7% | 1355 |
LB (Illumina) | 3,301,130 | 5,215 | 3,306,345 | 25.2% | 0.5 | 4,463 | 29.4% | 1313 |
LB-pH9 | 2,953,471 | 40,607 | 2,994,078 | 22.3% | 0.1 | 4,445 | 29.4% | 1308 |
LB-pH4 | 1,315,629 | 5,353 | 1,320,982 | 7.3% | 0.03 | 3,441 | 27.0% | 930 |
LB-15°C | 2,251,249 | 42,964 | 2,294,213 | 11.3% | 0.1 | 4,360 | 29.7% | 1292 |
LB-nitrite | 2,143,433 | 48,449 | 2,191,882 | 29.7% | 0.2 | 4,377 | 29.2% | 1277 |
LB-antibiotics | 718,712 | 3,615 | 722,327 | 14.0% | 0.03 | 2,892 | 27.1% | 785 |
LB-solid | 1,679,329 | 18,652 | 1,697,981 | 20.0% | 0.02 | 4,006 | 28.7% | 1150 |
minimal medium | 1,496,231 | 34,135 | 1,530,366 | 25.4% | 0.1 | 4,017 | 28.7% | 1151 |
spinach juice | 1,638,842 | 71,874 | 1,710,716 | 8.8% | 0.08 | 4,131 | 29.4% | 1215 |
radish sprouts (SOLiD) | 1,355,143 | 9,876 | 1,365,019 | 38.1% | 0.09 | 4,079 | 28.9% | 1180 |
radish sprouts (Illumina) | 3,724,713 | 31,160 | 3,755,873 | 60.3% | 0.29 | 3,852 | 26.8% | 1035 |
feces | 1,184,557 | 4,457 | 1,189,014 | 30.5% | 0.05 | 2,979 | 28.9% | 861 |
total | 25,752,765 | 362,854 | 26,115,619 | 26.4% | Ø 0.14 | 5,142 | 26.5% | 1621 |
1The number of mappable reads within each condition is listed for the genome and plasmid. The percentage of remaining rRNA is shown below.