Figure 1.

PCGF 1, 3, and 5 Variant PRC1 Complexes Catalyze H2AK119ub1 and Create A Polycomb Domain Containing PRC2 and H3K27me3

(A) A schematic illustrating the core components of canonical and variant PRC1 complexes.

(B) The TetO array at its integration site on mouse chromosome 8.

(C) Targeting of factors to the TetO via the TetR DNA-binding domain. Numbers represent qPCR primer positions (kb) with respect to TetO array.

(D) ChIP analysis for fusion protein occupancy (TetR), RING1B, H2AK119ub1, and histone H3 across the TetO containing locus. Fusion protein identity is indicated above each panel.

(E) As in (D) ChIP analysis for PRC2 components and H3K27me3. All ChIP experiments were performed at least in biological duplicate with error bars showing standard error of the mean (SEM).

See also Figure S1.