Table 1.

Primers, PCR conditions, and respective references

Primers	Sequence (5 $\mathbf{\text{'}}$ → 3')	Target	PCR condition			Amplicon (bp)	References
			Denaturin	Annealin	Extension
cadFU cadFR	TTGAAGGTAATTTAGATATG CTAATACCTAAAGTTGAAAC	cadF	94οC, 30 s	43οC, 30 s	72οC, 30 s	400	[11]
hipOU hipOR	GAAGAGGTTTGGGTGGTG AGCTAGCTTCGCATAATAACTTG	hipO	94οC, 30 s	53οC, 30 s	72οC, 30 s	735	[11]
aspU aspR	GGTATGATTTCTACAAAGCGAG ATAAAAGACTATCGTCGCGTG	asp	94οC, 30 s	53οC, 30 s	72οC, 30 s	500	[11]
flaAU flaAR	TTTCGTATTAACACAAATGGTGC CTGTAGTAATCTTAAAACATTTTG	flaA	94οC, 45 s	46οC, 45 s	72οC, 60 s	1743	this study
cdtjAU cdtjAR	AGGACTTGAACCTACTTTTC AGGTGGAGTAGTTAAAAACC	Cj-cdtA	94οC, 30 s	55οC, 30 s	72οC, 30 s	631	[31]
cdtj BU cdtjBR	ATCTTTTAACCTTGCTTTTGC GCAAGCATTAAAATCGCAGC	Cj-cdtB	94οC, 30 s	56οC, 30 s	72οC, 30 s	714	[31]
cdtjCU cdtjCR	TTTAGCCTTTGCAACTCCTA AAGGGGTAGCAGCTGTTAA	Cj-cdtC	94οC, 30 s	55οC, 30 s	72οC, 30 s	524	[31]
cdtCAU cdtCAR	ATTGCCAAGGCTAAAATCTC GATAAAGTCTCCAAAACTGC	Cc-cdtA	94οC, 30 s	55οC, 30 s	72οC, 30 s	329	[31]
cdtCBU cdtCBR	TTTAATGTATTATTTGCCGC TCATTGCCTATGCGTATG	Cc-cdtB	94οC, 30 s	56οC, 30 s	72οC, 30 s	413	[31]
cdtCCU cdtCCR	TAGGGATATGCACGCAAAAG GCTTAATACAGTTACGATAG	Cc-cdtC	94οC, 30 s	55οC, 30 s	72οC, 30 s	313	[31]
ciaBU ciaBR	TGCTAGCCATACTTAGGCGTTTT TTGATAATAGCGGACAATTTGAAA	ciaB	94οC, 30 s	54οC, 30 s	72οC, 30 s	610	this study
pldAU pldAR	AAGCTTATGCGTTTTT TATAAGGCTTTCTCC	PldA	94οC, 30 s	46οC, 30 s	72οC, 30 s	913	[32]
iamAU iamAR	GCGCAAAATATTATCACCC TTCACGACTACTATGCGG	iam	94οC, 30 s	47οC, 30 s	72οC, 30 s	518	[33]
virB11U virB11R	GAACAGGAAGTGGAAAAACTAGC TCCCGCATTGGGCTATATG	virB11	94οC, 30 s	52οC, 30 s	72οC, 120 s	708	[33]
ERICF ERICR	ATGTAAGCTCCTGGGGATTCA AAGTAAGTGACTGGGTGAGCG	ERIC	94οC, 30 s	52οC, 30 s	72οC, 300 s	variable	[34]