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Inhibition of reactive oxygen species generation. HT22 cells were treated with 10, 50, and 100 μM of 4,5-dimethoxypyrocatechol and then exposed to 2 mM glutamate for 8 h increased ROS production. Trolox (10 μM) was used as a positive control. Each bar represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. glutamate-injured cells (ANOVA)
