Figure 1.

Comparison of Act d 2 and dAct d 2 (TMSF-treated Act d 2). (A) Coomassie-stained immunodetection with serum pool from kiwi-allergic patients (serum pool), or with rabbit polyclonal antibodies against thaumatin-like proteins (anti-TLP), plant complex glycans (anti-N-gly), xylose or fucose (anti-xyl and anti-fuc). Act d 2 (Ad2) and dAct d 2 (dAd2). (B) Secondary structure composition of Act d 2 and dAct d 2, calculated from their far-ultraviolet circular dichroism data. (C) β-Glucanase activity as a function of carboxymethylated-Pachyman concentration in a Lineweaver–Burke plot. (D) Antifungal activity. Spores from Plectospharella cucumerina were grown under standard conditions. Time corresponds to days after inoculation. Growth was measured as absorbance at 660 nm.