TABLE 1.
Antibodies Used in This Study
| Antigen | Immunogen | Manufacturer, cat. no. | Host | Dilution |
|---|---|---|---|---|
| Agmatine (AGB) | Agmatine conjugated to bovine serum albumin |
Chemicon (Millipore); AB15681 |
Rb; polyclonal | 1:100 (IF); 1:800 (ICC) |
| Agmatine (AGB) | Agmatine cross-linked to purified fraction V bovine serum albumin with glutaraldehyde |
Abcam; ab626671 | Ch; polyclonal | 1:500 |
| Calretinin | Recombinant rat calretinin | Millipore; MAB1568 | Ms; monoclonal | 1:1,000 |
| Choline acetyl transferase (ChAT) |
Highly purified ChAT obtained from porcine brain |
Chemicon (Millipore); MAB5270 |
Ms; monoclonal | 1:500 |
| γ-Aminobutyric acid (GABA) | GABA conjugated to bovine serum albumin with glutaraldehyde |
Chemicon (Millipore); AB50161 |
Rb; polyclonal | 1:4,500 |
| Glutamate | Glutamate conjugated to bovine serum albumin with glutaraldehyde |
Chemicon (Millipore); AB50181 |
Rb; polyclonal | 1:4,500 |
| Glycine | Glycine conjugated to bovine serum albumin with glutaraldehyde |
Chemicon (Millipore); AB50201 |
Rb; polyclonal | 1:4,000 |
| Islet-1 | Truncated rat islet protein corresponding to amino acids 178-349 |
Developmental Studies Hybridoma Bank; 39.4D5 |
Ms; monoclonal | 1:200 |
| Neurokinin receptor 3 (NK3R) | Synthetic peptide (SSFISSPYTSSVDEYS) corresponding to amino acids 451-465 of rat NK-3 conjugated to bovine thyroglobulin |
Novus Biologicals; NB300-102 |
Rb; polyclonal | 1:4,000 |
| Nitric oxide synthase-brain (bNOS) |
Recombinant neuronal NOS fragment (amino acids 1-181) from rat brain |
Sigma-Aldrich; N2280 | Ms; monoclonal | 1:3,000 |
| Parvalbumin (PV) | Frog muscle parvalbumin | Sigma-Aldrich; P3088 | Ms; monoclonal | 1:500 |
| Protein kinase C-α (PKCα) | Purified bovine brain PKC | Sigma-Aldrich; P5704 | Ms; monoclonal | 1:400 |
| Protein kinase C-α (PKCα) | Synthetic peptide (KVNPQFVHPILQSAV) corresponding to amino acids 659-672 from the C-terminal variable region of rat PKCα |
Sigma-Aldrich; P4334 | Rb; monoclonal | 1:2,000 |
| Recoverin | Recombinant human recoverin |
Chemicon (Millipore); AB5585 |
Rb; polyclonal | 1:1,000 |
| Tyrosine hydroxylase (TH) | Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate) |
Chemicon (Millipore); AB152 |
Rb; polyclonal | 1:1,000 |
1
These antibodies were kindly donated by Dr. R.E. Marc, are licensed to Signature Immunologics, and are also commercially available through Abcam and/or Merck Millipore. Abbreviations: Rb, rabbit; Ch, chicken; Ms, mouse, IF, immunofluorescence, ICC, immunocytochemistry.