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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Comp Neurol. 2013 Aug 1;521(11):2416–2438. doi: 10.1002/cne.23305

TABLE 1.

Antibodies Used in This Study

Antigen Immunogen Manufacturer, cat. no. Host Dilution
Agmatine (AGB) Agmatine conjugated to
 bovine serum albumin
Chemicon (Millipore);
 AB15681
Rb; polyclonal 1:100 (IF);
1:800 (ICC)
Agmatine (AGB) Agmatine cross-linked to
 purified fraction V bovine
 serum albumin with
 glutaraldehyde
Abcam; ab626671 Ch; polyclonal 1:500
Calretinin Recombinant rat calretinin Millipore; MAB1568 Ms; monoclonal 1:1,000
Choline acetyl transferase
 (ChAT)
Highly purified ChAT
 obtained from porcine
 brain
Chemicon (Millipore);
 MAB5270
Ms; monoclonal 1:500
γ-Aminobutyric acid (GABA) GABA conjugated to bovine
 serum albumin with
 glutaraldehyde
Chemicon (Millipore);
 AB50161
Rb; polyclonal 1:4,500
Glutamate Glutamate conjugated to
 bovine serum albumin with
 glutaraldehyde
Chemicon (Millipore);
 AB50181
Rb; polyclonal 1:4,500
Glycine Glycine conjugated to
 bovine serum albumin with
 glutaraldehyde
Chemicon (Millipore);
 AB50201
Rb; polyclonal 1:4,000
Islet-1 Truncated rat islet protein
 corresponding to amino
 acids 178-349
Developmental Studies
 Hybridoma Bank; 39.4D5
Ms; monoclonal 1:200
Neurokinin receptor 3 (NK3R) Synthetic peptide
 (SSFISSPYTSSVDEYS)
 corresponding to amino
 acids 451-465 of rat
 NK-3 conjugated to
 bovine thyroglobulin
Novus Biologicals;
 NB300-102
Rb; polyclonal 1:4,000
Nitric oxide synthase-brain
 (bNOS)
Recombinant neuronal
 NOS fragment (amino
 acids 1-181) from rat
 brain
Sigma-Aldrich; N2280 Ms; monoclonal 1:3,000
Parvalbumin (PV) Frog muscle parvalbumin Sigma-Aldrich; P3088 Ms; monoclonal 1:500
Protein kinase C-α (PKCα) Purified bovine brain PKC Sigma-Aldrich; P5704 Ms; monoclonal 1:400
Protein kinase C-α (PKCα) Synthetic peptide
 (KVNPQFVHPILQSAV)
 corresponding to amino
 acids 659-672 from the
 C-terminal variable
 region of rat PKCα
Sigma-Aldrich; P4334 Rb; monoclonal 1:2,000
Recoverin Recombinant human
 recoverin
Chemicon (Millipore);
 AB5585
Rb; polyclonal 1:1,000
Tyrosine hydroxylase (TH) Denatured tyrosine
 hydroxylase from rat
 pheochromocytoma
 (denatured by sodium
 dodecyl sulfate)
Chemicon (Millipore);
 AB152
Rb; polyclonal 1:1,000
1

These antibodies were kindly donated by Dr. R.E. Marc, are licensed to Signature Immunologics, and are also commercially available through Abcam and/or Merck Millipore. Abbreviations: Rb, rabbit; Ch, chicken; Ms, mouse, IF, immunofluorescence, ICC, immunocytochemistry.