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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: J Immunol. 2014 Apr 14;192(10):4833–4843. doi: 10.4049/jimmunol.1400062

Figure 1. Monocyte expression of pro-inflammatory cytokines upon BCG stimulation of whole blood from newborns and infants.

Figure 1

(A) Monocyte expression of TNF-α, IL-6 and/or IL-12 from a representative infant at 36 weeks of age. Frequencies (%) of cells falling into each gate are shown for each plot. (B) Frequencies of monocyte subsets co-expressing pro-inflammatory cytokines upon BCG stimulation of whole blood from newborns, 10 and 36-week old infants (n = 25 for each group). (C) Frequencies of BCG-stimulated monocytes expressing all 3 pro-inflammatory cytokines (polyfunctional), any 2 of the 3 cytokines (bifunctional) or any 1 of the 3 cytokines (monofunctional). (D) Integrated median fluorescence intensity (iMFI) of BCG-induced TNF-α, IL-6 and IL-12 expression by monocytes, representing the product of the frequency and the MFI of cytokine+ monocytes, from newborns, 10 and 36-week old infants. For box and whisker plots, horizontal lines represent the median, boxes represent the interquartile range (IQR) and whiskers represent the range. Group comparisons were done using the Kruskal-Wallis test (Overall effect), followed by the Mann-Whitney test.