Fig. 1.

Iterative, hCD33-directed Library Synthesis and Screening for the Identification of Selective, High-affinity hCD33 Ligands. (a) Structures of the sialoside analogues that were constructed and screened. (b) Compiled results of the iterative microarray screening process for hCD33. In this process, screening with high and low concentrations of both hCD33 and the deposited sialoside allows for the identification of analogues that give increases in affinity. To aid the reader, key results, which were used to design the next library, are noted. (c) Counterscreening these libraries with related siglecs, hCD22 and mouse Siglec-1 (mSn), allows for the identification of ligands which also exhibit selectivity for hCD33. Shown are representative results from three independent experiments.