Table 3.
Sprouty/Tbx1 interactions during cardiovascular development
| E10.5a | E15.5-E17.5b | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Spry1 | Spry2 | Tbx1 | n | 1st PAA | PAA | PAA | Aortic arch | OFT defects | VSD |
| +/+ | +/+ | +/+ | 21 | 0 | 0 | 0 | nd | nd | nd |
| +/− | 17 | 1 | 0 | 0 | 0/38 | 0/38 | 1/17 | ||
| +/− | +/− | +/+ | 8 | 0 | 0 | 0 | 0/30 | 0/30 | 0/3 |
| +/− | 14 | 1 | 0 | 7* | 4/38 | 0/38 | 0/19 | ||
| −/− | +/− | +/+ | 10 | 0 | 0 | 1 | 0/20 | 0/20 | 0/9 |
| +/− | 22 | 1 | 1 | 17** | 9/33** | 0/33 | 0/23 | ||
| +/− | −/− | +/+ | 10 | 1 | 2 | 0 | 0/13 | 0/13 | 0/10 |
| +/− | 14 | 0 | 2 | 7* | 8/29**^ | 0/30 | 5/19 | ||
| −/− | −/− | +/+ | 16 | 0 | 6^ | 5^^ | 0/11 | 0/11 | 2/8 |
| +/− | 12 | 5** | 10* | 10*** | 12/16*** | 2/16 | 4/10 | ||
Aortic arch defects were scored at E10.5 by India ink injections (a) or at later stages by MRI or histology (b).
Outflow tract (OFT) defects and Ventricular Septal defects (VSD) were also scored in E15.5-E17.5 embryos.
Significant incidence compared to wildtype controls (Fisher's exact test, two tailed):
^
p=0.003
^^
p=0.01
Shaded cells indicate a significant difference between Tbx1+/+ and Tbx1+/− embryos:
*
p=0.02
**
p=0.01
**^
p=0.04
***
p<0.001