Figure 1. Myeloid-associated deletion of Mgat2 induces a spontaneous CVID-like condition.

Mgat2^wt/wt and Mgat2^ΔM/ΔM mice were immunized with ovalbumin (OVA) and alum. A) Serum anti-ova IgG end point titers identified a sub-set of Mgat2^ΔM/ΔM mice as low IgG responders (open squares). These low IgG responders did not induce detectable T cell responses to OVA as measured by B) cell proliferation and C) IL-5 production. D) Flow cytometry analysis of blood CD4⁺ T cells prior to vaccination showing the ratio of naïve (CD44^low) to effector/memory (CD44^hi) cells. E) The CD4⁺ T cell ratio phenotype was used to predict low IgG responders to Prevnar-13 vaccination, show as induced anti-polysaccharide IgG levels by ELISA at 1:50 serum dilution. (Eu, europium) *p<0.05, **p<0.01