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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Toxicol In Vitro. 2014 Jan 29;28(5):715–721. doi: 10.1016/j.tiv.2014.01.009

Fig 5.

Fig 5

Expression of oxidative stress genes in paraoxon-treated HSG cells. Cells were treated with either ethanol (0.1%) as a control or 10 μM PX for 4h (white bars) or 24 h (black bars). Fold-change measured by q-RT-PCR of gpx7, gss, gstm2, gstt2, sod2, and txnrd2 was measured. Expression was normalized to gapdh expression. Once data had been normalized (ΔCT), the average of 4 replicates in each group was used to calculate the ΔΔCt and fold change (fold-change = 2(ΔΔCT)). p-Values were calculated using ΔΔCt values, n=4, compared to the control using Student’s t-test. * represents p values < 0.05, ** represents p values < 0.01.