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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: J Immunol. 2014 May 16;192(12):5749–5760. doi: 10.4049/jimmunol.1303260

Figure 6. Frequencies of cDC, pDC, T- and NK-cell precursors in different subsets of PB HPCs and CD127 expression in subsets of in vitro-generated HPC.

Figure 6

(A) Representative flow cytograms and gating strategy for sorting cKithi, cKitlo, cKit+/hiCD7+, cKitloCD7+, and cKitloCD10+ subsets of PB HPCs and for CD127 expression in CD7+ PB HPCs (upper panels). PFs of cDCs, pDCs, T cells and NK cells were obtained by LDA of each HPC subsets using OP9-DL1 co-culture (lower panels).

(B) Flow cytometric analyses of CD127 expression (upper) in CD34+CD7−, CD34+CD7+, and CD34−CD7+ cells generated in four-week-bulk-culture of PB HPCs. CD5 and CD56 expressions were analyzed for the three HPC subsets (lower).