Table II.
Classification of T/NK-cell and DC potential of PB and CB HPC clones
| PB HPC precursor clone | Number of clones producing DC |
||||
|---|---|---|---|---|---|
| cDC and pDC | cDC | pDC | No DC | Total | |
| T and NK | 29b (49%) | 22b (24%) | 0 | 11(10)c | 62(61)c |
| T | 6b (10%) | 13b (14%) | 0 | 4(3)c | 23(22)c |
| NK | 9b (15%) | 10b (11%) | 0 | 67(63)c | 86(82)c |
|
| |||||
| T/NK subtotal | 44b (75%) | 45b (48%) | 0 | 82(76)c | 171(165)c |
| CD7+CD5−CD56− | 9b (16%) | 15b (16%) | 0 | 11(8)c | 35(32)c |
| Myeloid w/o CD7+ cellsa | 5 (9%) | 27 (29%) | 0 | 184 | 216 |
| DC alone | 0 | 8 (9%) | 0 | - | 8 |
|
| |||||
| Total | 58 (100%) | 95(100%) | 0 | 277 | 430 |
| CB HPC precursor clones | Number of clones producing DC |
||||
|---|---|---|---|---|---|
| cDC and pDC | cDC | pDC | No DC | Total | |
| T and NK | 134b (92%) | 63b (71%) | 0 | 3(3)c | 200(200)c |
| T | 7b (5%) | 16b (18%) | 1 | 13(12)c | 37(36)c |
| NK | 2b (1%) | 0 | 0 | 0 | 2(2)c |
|
| |||||
| T/NK subtotal | 143b (99%) | 79b (89%) | b1 | 16(15)c | 239(238)c |
| CD7+CD5−CD56− | 2b (1%) | 8b (9%) | 0 | 4(4)c | 14(14)c |
| Myeloid w/o CD7+ cellsa | 0 | 2 (2%) | 0 | 26 | 28 |
| DC alone | 0 | 0 | 0 | - | 0 |
|
| |||||
| Total | 145 (100%) | 89 (100%) | 1 | 46 | 281 |
Progenies from 430 and 281 clones in PB and CB HPCs were obtained by single cell sorting of CD34+Lin− cells from 9 and 3 donors, respectively. The DC potential was classified as cDC and/or pDC in each T and/or NK precursor clone.
a
The presence of myeloid cells including CD14+CD15− monocytic and/or CD14+CD15+ granulocytic cells but not CD7+ lymphoid cells (Fig. S3).
b
All clones producing both DCs and lymphoid cells gave rise to myeloid cells.
c
Number of clones accompanying monocyte and/or granulocyte progenies in parentheses.