Table III.
In vivo CD34+CD7+ and CD34+CD10+ clones | Number of clones producing DC |
||||
---|---|---|---|---|---|
cDC and pDC | cDC | pDC | No DC | Total | |
T and NK | 0 | 4 (4)a | 0 | 15 (3) | 19 (7) |
T | 0 | 4 (4) | 0 | 10 (0) | 14 (4) |
NK | 2 (1) | 1 (1) | 0 | 79 (17) | 82 (19) |
| |||||
T/NK subtotal | 2 (1) | 9 (9) | 0 | 104 (20) | 115 (30) |
CD7+CD5−CD56− | 0 | 3(3) | 0 | 5 (1) | 8 (4) |
Myeloid w/o CD7+ cells | 0 | 6 | 0 | 172 | 178 |
DC alone | 0 | 14 | 0 | - | 14 |
| |||||
Total | 2 | 32 | 0 | 281 | 315 |
CD34+Lin− CD7+ or CD34+Lin− CD10+ were sorted from 5 donors at single cell per well and cultured with OP9-DL1 cells for 4 to 5 weeks. The presence of T/NK/DC progenies was analyzed and classified, as noted in Table II. The difference in distribution of total DC-producer clones were significant by chi-square test (Yates' correction) (P = 0.002), based on the number of clones between the total T lineage-(T/NK dual lineage- plus T single lineage-phenotypes) and NK single lineage-phenotype (T: 8 producer clones vs 25 non-producer clones, NK: 3 producer clones vs 79 non-producer clones).
Number of clones accompanying monocyte and/or granulocyte progenies in parentheses.