Figure 5. Mutations in H2Av enhance the onset of mxc germline phenotypes and ectopic sex combs.

A-D. Male gonads from (A) wild-type and (B) mxc^G46 3^rd instar larvae appear wild-type, while the mxc mutant phenotype is detected at this stage in mxc^G46,H2Av^ΔCT;H2Av⁸¹⁰/TM6b (C) male larvae. Germ cells (Vasa⁺, green); hub (DE-cad⁺, red). (D, G) Testes from 1-day old mxc ^G46 adults, exhibit a combination of early germ cells and large germ cells that have initiated differentiaiton prematurely. Germ cells (Vasa⁺, green); hub (DE-cad⁺, blue); cyst cells (Tj⁺, red). (E, F, G) Testes from 1-day old mxc^G46,H2Av^ΔCT; H2Av⁸¹⁰/TM6b flies accumulate spermatogonia that appear to be stalled in S-phase, as indicated by EdU incorporation. (H) The L2 to L1 and L3 to L1 transformations are enhanced in mxc^G46 ;H2Av⁸¹⁰/TMb and mxc^G46, H2Av^ΔCT;H2Av⁸¹⁰/TM6b flies.