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. Author manuscript; available in PMC: 2014 Jun 8.
Published in final edited form as: J Immunol. 2008 Apr 1;180(7):4754–4762. doi: 10.4049/jimmunol.180.7.4754

Figure 4.

Figure 4

Histological and flow cytometric analysis of B6(CBA) → CD4+ T cell-depleted CBA 7 (n= 4) and 14 days (n=3) after transplantation. (A) Flow cytometric analysis of I-Ab and I-Ak expression on lung-resident live CD45+ cells in B6, CBA and B6(CBA) mice. Shaded curves represent isotype controls (B) H&E slides are represented at 400X magnification. (C) Rejection scores represented as mean ± S.E.M. Statistical analysis was conducted with the Student’s t test; B6(CBA) → CBA-CD4 (day7) vs. B6 → CBA-CD4 (day7) p<0.1, B6(CBA) → CBA-CD4 (day7) vs. B6(CBA) → CBA-CD4 (day14) p<0.1, B6(CBA) → CBA-CD4 (day14) vs. B6 → CBA-CD4 (day7) p>0.1 (D) Flow cytometric plots depict the percentage of live intragraft Thy1.2+ CD8+ cells that have the capacity to produce IFN-γ. Mean percentage ± S.E.M of IFN-γ producing Thy1.2+ CD8+ cells were 55.9 ± 9.3 and 62.9 ± 8.9 for days 7 and 14, respectively. Statistical analysis was conducted with the Student’s t test; B6(CBA) → CBA-CD4 (day7) vs. B6 → CBA-CD4 (day7) p<0.05, B6(CBA) → CBA-CD4 (day7) vs. B6(CBA) → CBA-CD4 (day14) p>0.1, B6(CBA) → CBA-CD4 (day14) vs. B6 → CBA-CD4 (day7) p>0.1.