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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: J Immunol. 2014 May 16;192(12):5776–5788. doi: 10.4049/jimmunol.1301661

FIGURE 4.

FIGURE 4

IL-1β and IL-6 can promote HA1-specific memory Th17 responses but fail to induce naïve CD4+ T cell differentiation into HA1-specific Th17. (A) Total CD4+ T cells were cultured for 7 days with 1 μg/ml anti-hDectin-1-HA1-loaded IFNDCs in the presence of different concentrations of Pam(3)CysSK(4). T cells were then restimulated for 48h with 1 μM pep 43 or pep 5 (control peptide). Cytokines in the supernatants were assessed. Error bars indicate mean±SD of triplicate assay. Two independent experiments showed similar results. (B) IL-1β and IL-6 levels in the culture supernatants of 1×105 IFNDCs incubated with 1 μg/ml anti-hDectin-1-HA1, 40 ng/ml Pam3CysSK4, or 1 μg/ml anti-hDectin-1-HA1 plus 40 ng/ml Pam(3)CysSK(4). Data are pooled from three independent experiments. (C and D) FACS-sorted naïve and memory CD4+ T cells were co-cultured for 7 days with IFNDCs (C) and blood mDCs (D) loaded with 1 μg/ml anti-hDectin-1-HA1 in the presence or absence of 40 ng/ml Pam(3)CysSK(4). T cells were restimulated for 48h with 1 μM peptides. The amount of IL-17 in the supernatants was assessed. In (C) and (D), background values generated with pep 5 were subtracted. P values were acquired by the Student’s t-test. Indications of donors and peptides tested in (C and D) are summarized in the table.