FIGURE 7.

Healthy individuals maintain influenza HA1-specific Th₁₇ that can be promoted by the combination of anti-hDectin-1-HA1 and TLR2 ligands. (A) Total CD4⁺ T cells were co-cultured for 7 days with IFNDCs loaded with 1 μg/ml anti-hDectin-1-HA1 in the presence or absence of indicated TLR ligands (40 ng/ml Pam(3)CysSK(4), 1μg/ml poly IC, 50 ng/ml flagellin, 500 ng/ml R848). T cells were then restimulated for 48h with 1 μM of indicated peptides. Cytokines in the culture supernatants were assessed. Two independent experiments showed similar results. (B and C) Total CD4⁺ T cells from healthy donors were co-cultured for 7 days with 1 μg/ml anti-hDectin-1-HA1-loaded IFNDCs in the presence or absence of 40 ng/ml Pam(3)CysSK(4) (B) or P. gingivalis LPS (C). T cells were then restimulated with 1 μM indicated peptides. Cytokines in the culture supernatants were assessed. (D) Total CD4⁺ T cells were co-cultured for 7 days with IFNDCs (top panels) or mDCs (lower panels) loaded with 1 μg/ml anti-hDectin-1-HA1 in the presence or absence of P. gingivalis LPS (40 ng/ml) or E. Coli LPS (100 ng/ml). T cells were then restimulated for 48h with 1 μM of indicated peptides. Cytokines in the culture supernatants were assessed. Two independent experiments showed similar results. In (A-D), Error bars indicate mean±SD of triplicate assay.