Fig. 7.

Stability of G805R-LDLR. CHO T-REx cells stably transfected with the WT-LDLR plasmid or the G805R-LDLR plasmid were incubated with tetracycline (tet) (1 μg/ml) to induce the expression of the transgenes. The media were removed and replaced with media without tetracycline and cultured for the indicated time intervals (h: hours), before Western blot analysis of cell lysates was performed using an antibody against the ligand-binding domain of the LDLR. Western blot of cells cultured in the absence of tetracycline to induce gene expression (No tet) was used as a control. One representative of three separate experiments is shown.