. Author manuscript; available in PMC: 2014 Jun 9.

Published in final edited form as: Front Biosci (Landmark Ed). 2014 Jun 1;19:967–985. doi: 10.2741/4261

Table 6.

Energy expenditure per kg non-fat mass in male Sprague-Dawley rats (Charles River Laboratories) fed a low-fat or high-fat diet between 4 and 13 weeks of age¹,²

Diet	VO₂ (L/kg non-fat mass/h)	VCO₂ (L/kg non-fat mass/h)	RQ (VCO₂/VO₂)	Heat production (Kcal/kg non-fat mass/h)
Low Fat	1.68 ± 0.020	1.53 ± 0.023^a	0.91 ± 0.004^a	8.16 ± 0.097
High Fat	1.73 ± 0.018	1.41 ± 0.011^b	0.82 ± 0.002^b	8.20 ± 0.072

See Tables 2 and 5 for experimental detail. At 13 weeks of age, rats were placed in a computer-controlled Oxymax instrument (an open circuit calorimeter; Columbus Instruments, OH) to measure 24-h O₂ consumption and CO₂ production (34). Heat production is calculated according to the Brouwer equation (61): HP (Kcal) = 3.82 × VO₂ (in L) + 1.15 × VCO₂ (in L).

Values are means ± SEM, n = 16 rats/diet group. Energy metabolism data are expressed per kg non-fat mass. Rat is the experimental unit in the statistical analysis.

^a,b

Means in a column without a common letter differ (P < 0.01), as analyzed by the unpaired t-test.