Figure 1. Convergent silencing of mCherry.

(A) Non-CT and CT constructs were designed using the Tol2 transgenesis system with the β-actin promoter driving sense transcription and an inverted CMV promoter driving antisense transcription of the mCherry open reading frame (see Supplement for maps and sequence information). All constructs harbored heart-specific GFP (cmlc2-GFP) which enabled identification of transgenic embryos. (B) Widespread expression of mCherry was observed in non-CT embryos (386 out of 386 transgenic embryos), whereas near complete loss of mCherry was observed in CT-mCherry embryos at 2 dpf (370 out of 402 transgenic embryos).