Skip to main content
. 2014 May 6;22(6):1084–1095. doi: 10.1038/mt.2014.52

Figure 5.

Figure 5

Stabilized huTRIM5α has potent human immunodeficiency virus (HIV)-1 restriction activity. (a,b) CD4 T cells expressing TRIM5α constructs fused to mCherry (mCherry-TRIM5α) or expressing TRIM5α as a distinct protein in an equimolar manner with GFP (GFP-T2A-TRIM5α) were infected with cell-free HIV-1SF162 (a) or HIV-1BK132 (b). After 8 days of culture, HIV-1 viral replication was measured by intracellular HIV-1GAG staining. (c) Mixed cultures where initially half the CD4 T cells are untransduced and half express mCherry-TRIM5α were infected with cell-free HIV-1SF162 and assayed after 8 days of culture for intracellular HIV-1GAG staining. Results are representative of those observed in three separate experiments performed in duplicate or larger replicates.