Skip to main content
. 2014 Feb 18;22(6):1151–1163. doi: 10.1038/mt.2014.5

Figure 5.

Figure 5

GL21.T-let in vivo selective targeting. (a, b) (nu/nu) Mice-bearing MCF-7 (Axl−)-Luc cells and A549 (Axl+)-Luc xenografts were injected intravenously either with 1,600 pmol of GL21.T or GL21.T-let conjugate. (a) After 24 hours tumors, kidney, liver, and spleen were excised, lysed for RNA extraction, and pooled for let-7g miRNA RT-qPCR. (b) After 24 or 48 hours A549 (Axl+)-Luc tumors were excised, lysed for protein or RNA extraction and pooled. Left panel: let-7g miRNA levels were quantified by RT-qPCR (middle panel), HMGA2 mRNA levels were quantified by RT-qPCR. Right panel: lysates were immunoblotted with anti-HMGA2, anti-caspase-3, and anti-αTub antibodies, as indicated. Band intensity was calculated as in Figure 1. Molecular weights of full-length and cleaved (active) caspase-3 are shown. In (a, b), error bars depict mean values of experimental replicates ± SD (n = 3). HMGA2, high mobility group AT-hook 2; mRNA, messenger RNA; miRNA, microRNA; RT-qPCR, reverse transcription quantitative polymerase chain reaction.