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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: J Immunol. 2014 May 7;192(12):5481–5489. doi: 10.4049/jimmunol.1302846

Figure 5. Cellular buffering capacity of Car4+/+ and Car4−/− eosinophils.

Figure 5

A, With > 90% purity, Car4+/+ and Car4−/− bone marrow–derived eosinophils (EOS, 2×106) were assessed for their cellular pH buffering capacity in saline without IL-5 following repetitive addition of 5 μL of 0.1 M HCl into 1 mL of cell suspension each time, with cell-free saline solution alone as negative control without cellular buffering. B, The intracellular pH changes in Car4+/+ and Car4−/− eosinophils were graphed before and after the exogenous lactate challenge (20 minutes), demonstrating no difference in buffering capacity which is required for lactate transport into cells. (mean ± SEM)