Fig. 2.

T cell polarization in lungs after MAV-1 infection. Mice were infected i.n. with MAV-1 or mock infected with conditioned media and lung lymphocytes were isolated at 7 dpi. A–C) Intracellular cytokine staining was used to quantify the percentage of CD4⁺ T cells that were IL-17⁺, IFN-γ⁺, or IL-4⁺. D–G) Lung leukocytes or mediastinal lymph node cells were stimulated overnight with anti-CD3 antibody and ELISA was used to measure IL-17 or IFN-γ production in the supernatant. Combined data from n=5 (A–C) and n=10 (D–G) mice per group are presented as means ± S.E.M. Statistical comparisons were made using Mann-Whitney test. **P<0.01 and ***P<0.001, comparing mock to MAV-1.