Fig. 3.

Cell types contributing to IL-17 and IFN-γ production after MAV-1 infection. Mice were infected i.n. with MAV-1 or mock infected with conditioned media and lung lymphocytes were isolated at 7 dpi. A) Lung leukocytes were stimulated with PMA/ionomycin and intracellular cytokine staining was used to quantify the percentage of each cell type that was IL-17⁺. B) Lung leukocytes were stimulated overnight with MAV-1-infected APCs and intracellular cytokine staining was used to quantify the percent of each cell type that was IL-17⁺. C) Lung leukocytes were stimulated with PMA/ionomycin and intracellular cytokine staining was used to quantify the percentage of each cell type that was IFN-γ⁺. Combined data from n=5 mice per group are presented as means ± S.E.M. Statistical comparisons were made using two-way ANOVA followed by Bonferroni’s multiple comparison tests. ***P<0.001, comparing mock to MAV-1.