Losartan (LOS) inhibits fractional NIC-evoked [3H]DA and [3H]NE release (top) and total [3H]DA and [3H]NE overflow (bottom) from rat striatal and hypothalamic slices, respectively. Arrow indicates the time point at which NIC (10 and 100 μM, for [3H]DA and [3H]NE release assays, respectively) was added to the superfusion buffer. Data are expressed as mean ± S.E.M. Fractional release data are expressed as a percentage of basal. Basal values for fractional [3H]DA and [3H]NE release were 0.76 ± 0.053 and 0.39 ± 0.028% tissue-[3H],respectively. n = 6 rats; **p < 0.01, indicates different from control (0 LOS; effect of NIC in the absence of LOS or MEC).