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. Author manuscript; available in PMC: 2014 Jun 9.
Published in final edited form as: J Biol Chem. 2007 Aug 18;282(44):31964–31971. doi: 10.1074/jbc.M705440200

Fig. 3.

Fig. 3

SDS-PAGE separation of NAG-NAM oligomers. Lane 1: oligomers generated from reaction of Lipid IV (2b, 110 μCi/μmol) under standard assay conditions (Experimental procedures) and quenched at 45 min. Lane 2: oligomers generated from reaction of 10 μM acetylated Lipid II (1c, 72 μCi/μmol) and A. aeolicus PBP1A PGT domain (3.3 μM) quenched at 3 min. The use of high enzyme: substrate ratios biases the product distribution to shorter oligomers, ≤ (NAG-NAM)20, but oligomers up to (NAG-NAM)30 can be resolved under these conditions (see Fig. S1).