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SDS-PAGE analysis of Lipid II (1b, 288 μCi/μmol) reactions catalyzed by the PGT domains of (A) A. aeolicus PBP1A and (B) E. coli PBP1A. Reactions were carried out as described in Experimental Procedures and quenched at the indicated time-points. Lane L: oligomer ladder; lane S: Lipid II (1, 2 pmol) and Lipid IV (2b, 2 pmol) run as standards.
