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. Author manuscript; available in PMC: 2014 Jun 9.
Published in final edited form as: J Biol Chem. 2007 Aug 18;282(44):31964–31971. doi: 10.1074/jbc.M705440200

Fig. 6.

Fig. 6

Radiolabeled oligomers incubated (+) or not (−) with 4 μM cold Lipid II (1a) and (A) 0.8 μM A. aeolicus PBP1A PGT domain or (B) 0.8 μM E. coli PBP1A. Lane 1: radiolabeled oligomeric starting material generated from 8 μM [14C]-Lipid IV (2b); lane 2: oligomeric starting material incubated with enzyme alone; lane 3: oligomeric starting material incubated with enzyme and 4 μM cold Lipid II (1a) for 10 min. New bands are indicated by arrows. (C) Control reaction using 4 μM radiolabeled ([14C]-GlcNAc)-Lipid II (1b) incubated with 0.4 μM full-length E. coli PBP1A under standard assay conditions for 5 min.