Figure 3.

Protein expression of GlyR Cl⁻ channel subunits in human and guinea pig airway smooth muscle detected by immunoblot. Representative gel images of immunoblot of known glycine subunits. Protein was extracted from native human airway smooth muscle, native guinea pig airway smooth muscle, and cultured immortalized human airway smooth muscle cells and probed with antibodies against GLRA1 and the GLRB subunits of GlyR Cl⁻ channels. Protein isolated from human whole brain and retinal lysates was used as positive controls. Gel images are representative of the analysis of 4 individual samples from native guinea pig airway smooth muscle, native human airway smooth muscle, and immortalized human airway smooth muscle cells. Lane A: left panel, guinea pig retina; right panel, guinea pig brain; lane B: native human airway smooth muscle; lane C: cultured immortalized human airway smooth muscle cells; and lane D: native guinea pig airway smooth muscle.