Figure 2. Adiposity drives leukocytosis.
A) Experimental overview: 1. WT or 2. Ob/Ob BM was transplanted into WT recipients. 3. WT or 4. Ob/Ob BM was transplanted into Ob/Ob recipients. BM was allowed to reconstitute for 5 weeks. B) Monocyte levels, C) BM progenitors and. D) cell cycle analysis of BM progenitors. All data mean ± SEM, n=5/group. *P<0.05.
E, F) WT and Ob/Ob mice were treated with a SGLT2i. E) Blood glucose levels. F) Blood leukocytes. All data mean ± SEM, n=5/group. *P<0.05.
G) Conditioned media from cultured lean or Ob/Ob VAT was incubated (10% v/v) with BM progenitor cells and GMP proliferation was measured by EdU incorporation. n=6 (independent conditioned media treatments from the VAT of 6 mice/group).
H) Experimental overview: FPT: Equal portions of VAT from lean or Ob/Ob mice were transplanted into lean WT recipients. I) Monocyte levels were monitored weekly for 4 weeks. J, K) After 4 weeks the J) abundance and K) cell cycle of the BM progenitor cells were determined by flow cytometry. All data mean ± SEM, n=5/group. *P<0.05.