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. Author manuscript; available in PMC: 2015 May 6.
Published in final edited form as: Cell Metab. 2014 May 6;19(5):821–835. doi: 10.1016/j.cmet.2014.03.029

Figure 6. TLR4/MyD88 signaling in CD11c+ ATMs drives leukocytosis in obesity.

Figure 6

A) Experimental outline: Ob/Ob mice were transplanted with BM from Myd88flx/flx, Myd88flx/flx LysM Cre or Myd88flx/flx CD11c Cre and allowed to reconstitute for 5 weeks. B) Schematic overview of the LysM and CD11c Cre induced deletion of Myd88 in the respective myeloid cells and the overlapping CD11c+ macrophage. C) Blood leukocyte levels, D) BM progenitor abundance and E) cell cycle analysis. All data mean ± SEM, n=6/group. *P<0.05.

F) Experimental overview: Ob/Ob mice were injected intraperitoneally with PBS or clodronate (CLO) liposomes (to deplete ATMs). The VAT was harvested washed and transplanted into lean WT mice for 4 weeks. G) Flow cytometry plots of the ATM subsets (parent gate CD45+), H) F4/80 staining of the VAT and I) F4/80 and Cd68 mRNA expression in the VAT. J) Circulating blood monocyte levels, K) BM progenitor abundance and L) proliferation after transplantation. All data mean ± SEM, n=5/group. *P<0.05. See also Figure S5A.