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. 2010 Feb;24(2):415–424. doi: 10.1096/fj.09-140566

Figure 2.

Figure 2

Functional effects of AdMYH6 gene transfer on healthy rabbit cardiac myocytes. AdMYH6 gene transfer potentiates sarcomere shortening, whereas the calcium transient is unaltered. All experiments were performed 48 h after gene transfer. A) Summary of AdMYH6 effects on contraction amplitude. AdMYH7 control values did not differ from nontransduced control values (53.9±5.23 nm, n=18 vs. 60.5±4.5 nm, n=74). AdMYH6 increased contraction amplitude regardless of the presence or location of the FLAG epitope. AdMYH6 3′FLAG amplitude = 85.8 ± 4.8 nm, n = 86; AdMYH6 5′FLAG amplitude = 83.1 ± 5.23 nm, n = 37; AdMYH6 no FLAG amplitude = 82.3 ± 7.7 nm, n = 19. B) Calcium transient amplitudes were the same between nontransduced control (0.057±0.005, n=38), AdMYH6 3′FLAG-treated (0.062±0.006, n=34), and AdMYH6 5′FLAG-treated (0.069±0.006, n=32) cardiac myocytes. C, D) Representative recordings of sarcomere length (SL) shortening (C) and intracellular calcium transients (D). *P < 0.05 vs. control (no virus); ∧P < 0.05 vs. AdMYH7 control; ANOVA with Tukey post hoc between-group comparisons.